RFX5 Polyclonal antibody

• Featured Product
• KD/KO Validated

RFX5 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 12137-1-AP

Synonyms

DNA binding protein RFX5, Regulatory factor X 5, RFX5

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Validation Data Gallery

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  WB analysis using 12137-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12137-1-AP (RFX5 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HeLa cells, Jurkat cells, K-562 cells, Raji cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below

Product Information

12137-1-AP targets RFX5 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RFX5 fusion protein Ag2783
• Full Name: regulatory factor X, 5 (influences HLA class II expression)
• Calculated Molecular Weight: 616 aa, 65 kDa
• Observed Molecular Weight: 65 kDa
• GenBank Accession Number: BC017471
• Gene Symbol: RFX5
• Gene ID (NCBI): 5993
• RRID: AB_2179039
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Sci Rep; RFX5 promotes the progression of hepatocellular carcinoma through transcriptional activation of KDM4A.; Authors - Dong-Bo Chen ; KO Validated
human	WB	Chin Med J (Engl); Regulatory factor X5 promotes hepatocellular carcinoma progression by transactivating tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein theta and suppressing apoptosis.; Authors - Dong-Bo Chen