RENALASE Monoclonal antibody

RENALASE Monoclonal Antibody for IHC, WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

Publications

2

CloneNo.

3A12F5

Cat no : 60128-1-Ig

Synonyms

C10orf59, MAO C, Monoamine oxidase C, RENALASE, RNLS

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Validation Data Gallery

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  WB analysis of human heart using 60128-1-Ig

  human heart tissue were subjected to SDS PAGE followed by western blot with 60128-1-Ig (RENALASE antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 60128-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney slide using 60128-1-Ig (RENALASE Antibody) at dilution of 1:50.

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  IHC staining of human kidney using 60128-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney slide using 60128-1-Ig (RENALASE Antibody) at dilution of 1:50.

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  IHC staining of human skeletal muscle using 60128-1-Ig

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle slide using 60128-1-Ig (RENALASE Antibody) at dilution of 1:50.

• 

  IHC staining of human skeletal muscle using 60128-1-Ig

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle slide using 60128-1-Ig (RENALASE Antibody) at dilution of 1:50.

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Tested Applications

• Positive WB detected in: human heart tissue
• Positive IHC detected in: human kidney tissue, human skeletal muscle tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

60128-1-Ig targets RENALASE in WB, IHC,ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: RENALASE fusion protein Ag13061
• Full Name: chromosome 10 open reading frame 59
• Calculated Molecular Weight: 38 kDa
• Observed Molecular Weight: 38 kDa
• GenBank Accession Number: BC005364
• Gene Symbol: C10orf59
• Gene ID (NCBI): 55328
• RRID: AB_2034903
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RNLS, also named as Renalase, C10orf59 and MAO-C, belongs to the renalase family. It is probable FAD-dependent amine oxidase secreted by the kidney, which circulates in blood and modulates cardiac function and systemic blood pressure. RNLS degrades catecholamines such as dopamine, norepinephrine and epinephrine in vitro. It lowers blood pressure in vivo by decreasing cardiac contractility and heart rate and preventing a compensatory increase in peripheral vascular tone, suggesting a causal link to the increased plasma catecholamine and heightened cardiovascular risk. High concentrations of catecholamines activate plasma renalase and promotes its secretion and synthesis. RNLS has physiologically relevant catecholamine-oxidizing activity. (PMID:15841207 ) This antibody is specific to RNLS.

Publications

Species	Application	Title
human	WB	Nephrol Dial Transplant; Renalase in hypertension and kidney disease.; Authors - Desir Gary V GV
human	WB	J Am Soc Hypertens; Human renalase: a review of its biology, function, and implications for hypertension.; Authors - Desir Gary V GV