RENALASE Polyclonal antibody

• Featured Product
• KD/KO Validated

RENALASE Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat, mouse

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

7

Cat no : 15003-1-AP

Synonyms

C10orf59, MAO C, Monoamine oxidase C, RENALASE, RNLS

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Validation Data Gallery

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  WB analysis using 15003-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15003-1-AP (RENALASE antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 15003-1-AP

  IP result of anti-RENALASE (IP:15003-1-AP, 4ug; Detection:15003-1-AP 1:500) with HEK-293 cells lysate 2000ug.

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  IHC staining of human kidney using 15003-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 15003-1-AP (RENALASE antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human kidney using 15003-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 15003-1-AP (RENALASE antibody) at dilution of 1:100 (under 10x lens).

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  IF Staining of mouse kidney using 15003-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse kidney tissue using RENALASE antibody (15003-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HEK-293 using 15003-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using 15003-1-AP (RENALASE antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: Caco-2 cells
• Positive IP detected in: HEK-293 cells
• Positive IHC detected in: human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: mouse kidney tissue, HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 7 publications below
• IHC: See 2 publications below
• IF: See 2 publications below

Product Information

15003-1-AP targets RENALASE in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, rat, mouse samples.

• Tested Reactivity: human, rat, mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RENALASE fusion protein Ag13061
• Full Name: chromosome 10 open reading frame 59
• Calculated Molecular Weight: 38 kDa
• Observed Molecular Weight: 35 kDa
• GenBank Accession Number: BC005364
• Gene Symbol: C10orf59
• Gene ID (NCBI): 55328
• RRID: AB_1557298
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RNLS, also named as Renalase, C10orf59 and MAO-C, belongs to the renalase family. It is probable FAD-dependent amine oxidase secreted by the kidney, which circulates in blood and modulates cardiac function and systemic blood pressure. RNLS degrades catecholamines such as dopamine, norepinephrine and epinephrine in vitro. It lowers blood pressure in vivo by decreasing cardiac contractility and heart rate and preventing a compensatory increase in peripheral vascular tone, suggesting a causal link to the increased plasma catecholamine and heightened cardiovascular risk. High concentrations of catecholamines activate plasma renalase and promotes its secretion and synthesis. RNLS has physiologically relevant catecholamine-oxidizing activity. (PMID:15841207 ) This antibody is specific to RNLS.

Publications

Species	Application	Title
human,rat	WB,IHC	Circulation; Catecholamines regulate the activity, secretion, and synthesis of renalase.; Authors - Li Guoyong G
human,rat	WB,IHC	J Clin Invest; Renalase is a novel, soluble monoamine oxidase that regulates cardiac function and blood pressure.; Authors - Xu Jianchao J
mouse	WB	Kidney Int; Renalase deficiency aggravates ischemic myocardial damage.; Authors - Wu Yanling Y ; KO Validated
rat	WB,IF	Front Cardiovasc Med; Aerobic Exercise Training Improves Renal Injury in Spontaneously Hypertensive Rats by Increasing Renalase Expression in Medulla.; Authors - Minghao Luo
human	WB,IF	Life Sci; Renalase is localized to the small intestine crypt and expressed upon the activation of NF-κB p65 in mice model of fasting-induced oxidative stress.; Authors - Kai Aoki
human	WB	Exp Biol Med (Maywood); Plasma and urine renalase levels and activity during the recovery of renal function in kidney transplant recipients.; Authors - Janete Quelhas-Santos