REC8 Polyclonal antibody

REC8 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 10793-1-AP

Synonyms

Cohesin Rec8p, HR21spB, REC8, REC8 homolog (yeast), REC8L1, Rec8p



Tested Applications

Positive WB detected inhuman testis tissue, K-562 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10793-1-AP targets REC8 in WB, IF, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen REC8 fusion protein Ag1250
Full Name REC8 homolog (yeast)
Calculated Molecular Weight 63 kDa
Observed Molecular Weight 85-95 kDa
GenBank Accession NumberBC010887
Gene Symbol REC8
Gene ID (NCBI) 9985
RRIDAB_2178282
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

REC8, also named as Rec8p and REC8L1, belongs to the rad21 family. This cohesin protein is required during meiosis for separation of sister chromatids and homologous chromosomes. Proteolytic cleavage of REC8 on chromosome arms by separin during anaphase I allows for homologous chromosome separation in meiosis I and cleavage of REC8 on centromeres during anaphase II allows for sister chromatid separation in meiosis II. Rec8 is present in an unphosphorylated form early in meiotic prophase but is phosphorylated prior to meiosis I. This antibody is a rabbit polyclonal antibody raised against residues near the C terminus of human REC8. This antibody can recognize the monomeric, non-phosphorylated form(63-70kd) of REC8 and other forms(80-100kd) which represent either posttranslationally modified forms or alternative transcript products (PMID: 12615909,19463812).

Protocols

Product Specific Protocols
WB protocol for REC8 antibody 10793-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Clin Cancer Res

Ectopic Expression of Cancer-Testis Antigens in Cutaneous T-cell Lymphoma Patients.

Authors - Ivan V Litvinov
mouseIF

Cell Cycle

Knockout of cyclin B1 in granulosa cells causes female subfertility.

Authors - Jinmei Cheng

Cell Cycle

Elevated intracellular pH appears in aged oocytes and causes oocyte aneuploidy associated with the loss of cohesion in mice.

Authors - Jin-Mei Cheng
mouse,ratWB

Biomed Pharmacother

Ephedra herb reduces adriamycin-induced testicular toxicity by upregulating the gonadotropin-releasing hormone signalling pathway.

Authors - Yang Fu
humanIF

Int J Mol Sci

"Mitotic Slippage" and Extranuclear DNA in Cancer Chemoresistance: A Focus on Telomeres.

Authors - Kristine Salmina
humanWB

Exp Cell Res

The role of meiotic cohesin REC8 in chromosome segregation in gamma irradiation-induced endopolyploid tumour cells.

Authors - Erenpreisa Jekaterina J