RBM5 Polyclonal antibody

RBM5 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 19930-1-AP

Synonyms

G15, H37, LUCA15, Protein G15, RBM5, RMB5, RNA binding motif protein 5, RNA binding protein 5

---

Validation Data Gallery

• 

  WB analysis of PC-3 using 19930-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 19930-1-AP (RBM5 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 19930-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 19930-1-AP (RBM5 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 19930-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 19930-1-AP (RBM5 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

---

Tested Applications

• Positive WB detected in: PC-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below

Product Information

The immunogen of 19930-1-AP is RBM5 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RBM5 fusion protein Ag13908
• Full Name: RNA binding motif protein 5
• Calculated Molecular Weight: 815 aa, 92 kDa
• Observed Molecular Weight: 110-115 kDa
• GenBank Accession Number: BC002957
• Gene Symbol: RBM5
• Gene ID (NCBI): 10181
• RRID: AB_2878625
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RBM5, also named as RNA-binding protein 5, is a 815 amino acid protein, which contains 2 RRM (RNA recognition motif) domains and Belongs to the RBM5/RBM10 family. RBM5 localizes in the nucleus and is a component of the spliceosome A complex. RBM5 regulates alternative splicing of a number of mRNAs and may modulate splice site pairing after recruitment of the U1 and U2 snRNPs to the 5' and 3' splice sites of the intron. RBM5 may both positively and negatively regulate aopotosis by regulating the alternative splicing of several genes involved in this process, including FAS and CASP2/caspase-2. The phosphorylation of RBM5 protein would influence the detected molecular weight. A 105-110 KDa band was also reported (PMID: 27957556).

Publications

Species	Application	Title
human	WB	J Biol Chem; CHIP suppresses the proliferation and migration of A549 cells by mediating the ubiquitination of eIF2α and upregulation of tumor suppressor RBM5; Authors - Bo Jin
mouse	WB	J Neurotrauma; RNA Binding Motif 5 Gene Deletion Modulates Cell Signaling in a Sex-Dependent Manner but not Hippocampal Cell Death.; Authors - Jeffrey Farooq
mouse	WB	Neuroscience; Identification of Novel Targets of RBM5 in the Healthy and Injured Brain.; Authors - Travis C Jackson
mouse	WB	Cell Death Discov; Neuronal RBM5 modulates cell signaling responses to traumatic and hypoxic-ischemic injury in a sex-dependent manner; Authors - Kara Snyder