CoraLite®594-conjugated RBM39 Monoclonal antibody

RBM39 Monoclonal Antibody for IF, FC (Intra)

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rat

Applications

IF, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

2D2C8

Cat no : CL594-67420

Synonyms

CAPER, CAPERalpha, CC1.3, DKFZp781C0423, FLJ44170, fSAP59, HCC1, RBM39, RNA binding motif protein 39, RNA binding protein 39, RNPC2, Splicing factor HCC1

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Validation Data Gallery

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  IF Staining of HepG2 using CL594-67420

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite®594 RBM39 antibody (CL594-67420, Clone: 2D2C8 ) at dilution of 1:500.

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  FC experiment of HepG2 using CL594-67420

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human RBM39 (CL594-67420, Clone:2D2C8) (red), or 0.4 ug Mouse IgG2b Isotype Control (CL594-66360-3, Clone: K11B8C4B5) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

• Positive IF detected in: HepG2 cells
• Positive FC detected in: HepG2 cells

Recommended dilution

• Immunofluorescence (IF): IF : 1:250-1:1000
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-67420 targets RBM39 in IF, FC (Intra) applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: RBM39 fusion protein Ag15766
• Full Name: RNA binding motif protein 39
• Calculated Molecular Weight: 530 aa, 59 kDa
• Observed Molecular Weight: 60-66 kDa
• GenBank Accession Number: BC141835
• Gene Symbol: RBM39
• Gene ID (NCBI): 9584
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RBM39, also named as HCC1 or RNPC2, is a 530 amino acid protein, which contains three RRM (RNA recognition motif) domains and belongs to the splicing factor SR family. RBM39 is widely expressed in various tissues and with highly expression in pancreas, skeletal muscle, lung and brain. RBM39 is a transcriptional coactivator for steroid nuclear receptors ESR1/ER-alpha and ESR2/ER-beta, and JUN/AP-1. RBM39 may be involved in pre-mRNA splicing process.