RBM28 Polyclonal antibody

RBM28 Polyclonal Antibody for WB, IP, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 16484-1-AP

Synonyms

RBM28, RNA binding motif protein 28, RNA binding protein 28

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Validation Data Gallery

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  WB analysis of NIH/3T3 using 16484-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 16484-1-AP (RBM28 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IP experiment of NIH/3T3 using 16484-1-AP

  IP result of anti-RBM28 (IP:16484-1-AP, 4ug; Detection:16484-1-AP 1:1000) with NIH/3T3 cells lysate 1120 ug.

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  IF Staining of NIH/3T3 using 16484-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed NIH/3T3 cells using 16484-1-AP (RBM28 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: NIH/3T3 cells
• Positive IP detected in: NIH/3T3 cells
• Positive IF detected in: NIH/3T3 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IF: See 1 publications below

Product Information

16484-1-AP targets RBM28 in WB, IP, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RBM28 fusion protein Ag9606
• Full Name: RNA binding motif protein 28
• Calculated Molecular Weight: 756 aa, 86 kDa
• Observed Molecular Weight: 86 kDa
• GenBank Accession Number: BC013889
• Gene Symbol: RBM28
• Gene ID (NCBI): 55131
• RRID: AB_2238116
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB,IF	J Biol Chem; RNA binding protein RBM28 can translocate from the nucleolus to the nucleoplasm to inhibit the transcriptional activity of p53.; Authors - Xin Lin