RASSF7 Polyclonal antibody

RASSF7 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, Rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 21753-1-AP

Synonyms

C11orf13, HRAS1, HRC1, RASSF7

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Validation Data Gallery

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  WB analysis using 21753-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21753-1-AP (RASSF7 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 21753-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 21753-1-AP (RASSF7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 21753-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 21753-1-AP (RASSF7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 21753-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 21753-1-AP (RASSF7 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human thyroid cancer using 21753-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 21753-1-AP (RASSF7 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human thyroid cancer using 21753-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 21753-1-AP (RASSF7 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HL-60 cells, HepG2 cells, PC-12 cells
• Positive IHC detected in: human lung cancer tissue, human thyroid cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

21753-1-AP targets RASSF7 in WB, IHC, ELISA applications and shows reactivity with human, Rat samples.

• Tested Reactivity: human, Rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RASSF7 fusion protein Ag16123
• Full Name: Ras association (RalGDS/AF-6) domain family (N-terminal) member 7
• Calculated Molecular Weight: 373 aa, 40 kDa
• Observed Molecular Weight: 40 kDa
• GenBank Accession Number: BC106921
• Gene Symbol: RASSF7
• Gene ID (NCBI): 8045
• RRID: AB_10858387
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RASSF7, previously known as HRC1 (HRAS1 cluster 1) and C11orf13, is a member of the N-terminal RASSF family. RASSF7 protein localises to the centrosome and plays a key role in mitosis. RASSF7 is expressed in a broad range of different cell types and that this expression could be enhanced following exposure to hypoxia. RASSF7 has three isoforms with the MW 40 kDa, 36 kDa and 35 kDa.