RASIP1 Polyclonal antibody

RASIP1 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 26064-1-AP

Synonyms

RAIN, Ras interacting protein 1, RASIP1

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Validation Data Gallery

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  WB analysis of mouse lung using 26064-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 26064-1-AP (RASIP1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 26064-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 26064-1-AP (RASIP1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human heart using 26064-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 26064-1-AP (RASIP1 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human heart using 26064-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 26064-1-AP (RASIP1 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human lung using 26064-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 26064-1-AP (RASIP1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung using 26064-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 26064-1-AP (RASIP1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 26064-1-AP

  Immunofluorescent analysis of HepG2 cells using 26064-1-AP (RASIP1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse lung tissue, NIH/3T3 cells
• Positive IHC detected in: human heart tissue, human lung tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 26064-1-AP is RASIP1 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RASIP1 fusion protein Ag22036
• Full Name: Ras interacting protein 1
• Calculated Molecular Weight: 963 aa, 103 kDa
• Observed Molecular Weight: 115 kDa
• GenBank Accession Number: BC021860
• Gene Symbol: RASIP1
• Gene ID (NCBI): 54922
• RRID: AB_2880359
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RASIP1, also named as Ras-interacting protein 1 or Rain, is a 963 amino acid protein, which contains one dilute domain and one Ras-associating domian. RASIP1 is highly expressed in heart and detected at lower levels in placenta and pancreas. RASIP1 is required for the proper formation of vascular structures that develop via both vasculogenesis and angiogenesis. RASIP1 acts as a critical and vascular-specific regulator of GTPase signaling, cell architecture, and adhesion, which is essential for endothelial cell morphogenesis and blood vessel tubulogenesis.