RASIP1 Polyclonal antibody

RASIP1 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 17971-1-AP

Synonyms

RAIN, Ras interacting protein 1, RASIP1

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Validation Data Gallery

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  WB analysis using 17971-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17971-1-AP (RASIP1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse lung using 17971-1-AP

  IP result of anti-RASIP1 (IP:17971-1-AP, 4ug; Detection:17971-1-AP 1:500) with mouse lung tissue lysate 4000ug.

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  IHC staining of human lung using 17971-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 17971-1-AP (RASIP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung using 17971-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 17971-1-AP (RASIP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 17971-1-AP

  Immunofluorescent analysis of HepG2 cells using 17971-1-AP (RASIP1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse lung tissue, rat lung tissue
• Positive IP detected in: mouse lung tissue
• Positive IHC detected in: human lung tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IF: See 2 publications below

Product Information

17971-1-AP targets RASIP1 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RASIP1 fusion protein Ag11982
• Full Name: Ras interacting protein 1
• Calculated Molecular Weight: 963 aa, 103 kDa
• Observed Molecular Weight: 115 kDa
• GenBank Accession Number: BC028614
• Gene Symbol: RASIP1
• Gene ID (NCBI): 54922
• RRID: AB_2878474
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RASIP1, also named as Ras-interacting protein 1 or Rain, is a 963 amino acid protein, which contains one dilute domain and one Ras-associating domian. RASIP1 is highly expressed in heart and detected at lower levels in placenta and pancreas. RASIP1 is required for the proper formation of vascular structures that develop via both vasculogenesis and angiogenesis. RASIP1 acts as a critical and vascular-specific regulator of GTPase signaling, cell architecture, and adhesion, which is essential for endothelial cell morphogenesis and blood vessel tubulogenesis.

Publications

Species	Application	Title
mouse	IF	Arterioscler Thromb Vasc Biol; Adventitial Sca1+ Cells Transduced With ETV2 Are Committed to the Endothelial Fate and Improve Vascular Remodeling After Injury.; Authors - Alexandra Le Bras
mouse	WB	Neuroscience; Hypoxic Postconditioning Promotes Angiogenesis After Ischemic Stroke; Authors - Wenjie Shi
mouse	IF	Inflamm Regen; A Sox17 downstream gene Rasip1 is involved in the hematopoietic activity of intra-aortic hematopoietic clusters in the midgestation mouse embryo; Authors - Gerel Melig