RAP1A Monoclonal antibody

RAP1A Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

CloneNo.

1G12D8

Reviews (1)

Cat no : 68125-1-Ig

Synonyms

C21KG, G 22K, GTP binding protein smg p21A, KREV 1, KREV1, RAP1, RAP1A, Ras related protein Krev 1, Ras related protein Rap 1A, SMGP21

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Validation Data Gallery

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  WB analysis using 68125-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68125-1-Ig (RAP1A antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

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  IHC staining of human liver cancer using 68125-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 68125-1-Ig (RAP1A antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 68125-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 68125-1-Ig (RAP1A antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HEK-293 cells, LNCaP cells, HeLa cells, Jurkat cells, MOLT-4 cells, K-562 cells, Raji cells, HSC-T6 cells, NIH/3T3 cells
• Positive IHC detected in: human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

68125-1-Ig targets RAP1A in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: RAP1A fusion protein Ag9463
• Full Name: RAP1A, member of RAS oncogene family
• Calculated Molecular Weight: 184 aa, 21 kDa
• Observed Molecular Weight: 21 kDa
• GenBank Accession Number: BC014086
• Gene Symbol: RAP1A
• Gene ID (NCBI): 5906
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RAP1A(Ras-related protein Rap-1A), also known as KREV1. Induces morphological reversion of a cell line transformed by a Ras oncogene. The biological roles of RAP1A include inducing neurite outgrowth and embryonic angiogenesis(PMID: 17916086). As one member of RAS oncogene family, it is a widely expressed receptor,with high mRNA expression in many tissues, particularly abundant in the colon. In cells, RAP1A was localized in cytoplasm and cell membrane(PMID: 11359771, PMID: 11359771).

Publications

Species	Application	Title
mouse	WB	Autoimmunity; RGS7 silence protects palmitic acid-induced pancreatic β-cell injury by inactivating the chemokine signaling pathway; Authors - Yurong Zhu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Guorong (Verified Customer) (11-22-2022)

Multiple bands distributed between 50-150 kDa were observed . Does not represent the performance in other kind of samples

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: ARPE-19

RAP1A Antibody Western Blot validation (1:2000 dilution) in ARPE-19 (Cat no:68125-1-Ig)