RANBP10 Polyclonal antibody

• Featured Product
• KD/KO Validated

RANBP10 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 21107-1-AP

Synonyms

KIAA1464, RAN binding protein 10, RANBP10

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Validation Data Gallery

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  WB analysis of MCF-7 using 21107-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 21107-1-AP (RANBP10 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 21107-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 21107-1-AP (RANBP10 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 21107-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 21107-1-AP (RANBP10 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of LNCaP using 21107-1-AP

  LNCaP cells were subjected to SDS PAGE followed by western blot with 21107-1-AP (RANBP10 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 21107-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 21107-1-AP (RANBP10 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human hepatocirrhosis using 21107-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 21107-1-AP (RANBP10 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human hepatocirrhosis using 21107-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 21107-1-AP (RANBP10 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human heart using 21107-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 21107-1-AP (RANBP10 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 21107-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 21107-1-AP (RANBP10 antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: MCF-7 cells, mouse skeletal muscle tissue, mouse heart tissue, LNCaP cells, mouse brain tissue
• Positive IHC detected in: human hepatocirrhosis tissue, human heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IF: See 1 publications below

Product Information

The immunogen of 21107-1-AP is RANBP10 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RANBP10 fusion protein Ag15387
• Full Name: RAN binding protein 10
• Calculated Molecular Weight: 620 aa, 67 kDa
• Observed Molecular Weight: 67-70 kDa
• GenBank Accession Number: BC099917
• Gene Symbol: RANBP10
• Gene ID (NCBI): 57610
• RRID: AB_10732603
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB,IF	Theranostics; miR-196a Enhances Neuronal Morphology through Suppressing RANBP10 to Provide Neuroprotection in Huntington's Disease.; Authors - Lu-Shiun Her
human	WB	PLoS Pathog; A Ran-binding protein facilitates nuclear import of human papillomavirus type 16.; Authors - Kun-Yi Lai ; KD Validated