CoraLite® Plus 488-conjugated RALY Monoclonal antibody

RALY Monoclonal Antibody for IF, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

IF, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2B10C3

Cat no : CL488-68011

Synonyms

Autoantigen p542, hnRNP core protein C like 2, HNRPCL2, P542, RALY, RNA binding protein Raly

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Validation Data Gallery

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  IF Staining of A431 using CL488-68011

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using CoraLite® Plus 488 RALY antibody (CL488-68011, Clone: 2B10C3 ) at dilution of 1:600.

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  FC experiment of A431 using CL488-68011

  1X10^6 A431 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human RALY (CL488-68011, Clone:2B10C3) (red), or 0.4 ug CoraLite® Plus 488 Mouse IgG1 Isotype Control (MOPC-21) (CL488-65124, Clone: MOPC-21) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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  FC experiment of A431 using CL488-68011

  1X10^6 A431 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human RALY (CL488-68011, Clone:2B10C3) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

• Positive IF detected in: A431 cells
• Positive FC detected in: A431 cells

Recommended dilution

• Immunofluorescence (IF): IF : 1:300-1:1200
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-68011 targets RALY in IF, FC (Intra) applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: RALY fusion protein Ag12250
• Full Name: RNA binding protein, autoantigenic (hnRNP-associated with lethal yellow homolog (mouse))
• Calculated Molecular Weight: 307 aa, 33 kDa
• Observed Molecular Weight: 37 kDa
• GenBank Accession Number: BC103753
• Gene Symbol: RALY
• Gene ID (NCBI): 22913
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RNA-binding protein RALY is a protein that in humans is encoded by the RALY gene. In infectious mononucleosis, anti-EBNA-1 antibodies are produced which cross-react with multiple normal human proteins. The cross-reactivity is due to anti-gly/ala antibodies that cross-react with host proteins containing configurations like those in the EBNA-1 repeat. One such antigen is RALY which is a member of the heterogeneous nuclear ribonucleoprotein gene family.