RALBP1 Monoclonal antibody

RALBP1 Monoclonal Antibody for WB, FC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse

Applications

WB, FC, ELISA

Conjugate

Unconjugated

CloneNo.

1B2F5

Cat no : 67359-1-Ig

Synonyms

76 kDa Ral interacting protein, DNP SG ATPase, Ral interacting protein 1, ralA binding protein 1, RALBP1, RIP1, RLIP1, RLIP76

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Validation Data Gallery

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  WB analysis of NIH/3T3 using 67359-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67359-1-Ig (RALBP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 67359-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 67359-1-Ig (RALBP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 67359-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 67359-1-Ig (RALBP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of LNCaP using 67359-1-Ig

  LNCaP cells were subjected to SDS PAGE followed by western blot with 67359-1-Ig (RALBP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 67359-1-Ig

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 67359-1-Ig (RALBP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  FC experiment of HEK-293 using 67359-1-Ig

  1X10^6 HEK-293 cells were stained with 0.20 ug/test Anti-Human RALBP1 (67359-1-Ig, Clone:1B2F5) (red) or 0.20 ug control antibody (blue) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) with dilution 1:1000. Fixed with90% MeOH.

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Tested Applications

• Positive WB detected in: NIH/3T3 cells, Jurkat cells, HEK-293 cells, LNCaP cells, MCF-7 cells
• Positive FC detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

67359-1-Ig targets RALBP1 in WB, FC, ELISA applications and shows reactivity with Human, mouse samples.

• Tested Reactivity: Human, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: RALBP1 fusion protein Ag28618
• Full Name: ralA binding protein 1
• Calculated Molecular Weight: 655 aa, 76 kDa
• Observed Molecular Weight: 95 kDa
• GenBank Accession Number: BC013126
• Gene Symbol: RALBP1
• Gene ID (NCBI): 10928
• RRID: AB_2882611
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.