CoraLite®594-conjugated RALA Monoclonal antibody

RALA Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1H9H5

Cat no : CL594-67093

Synonyms

RAL, RALA, Ras related protein Ral A

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Validation Data Gallery

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  FC experiment of MCF-7 using CL594-67093

  1X10^6 MCF-7 cells were intracellularly stained with 0.8 ug CoraLite®594 Anti-Human RALA (CL594-67093, Clone:1H9H5) (red), or 0.8 ug CoraLite®594 Mouse IgG2a Isotype Control (C1.18.4) (CL594-65208, Clone: C1.18.4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive FC detected in: MCF-7 cells

Recommended dilution

• Flow Cytometry (FC): FC : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-67093 targets RALA in FC (Intra) applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: RALA fusion protein Ag5329
• Full Name: v-ral simian leukemia viral oncogene homolog A (ras related)
• Calculated Molecular Weight: 206 aa, 24 kDa
• Observed Molecular Weight: 24 kDa
• GenBank Accession Number: BC039858
• Gene Symbol: RALA
• Gene ID (NCBI): 5898
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.