RAI14 Polyclonal antibody

• Featured Product
• KD/KO Validated

RAI14 Polyclonal Antibody for WB, IP, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

9

Cat no : 17507-1-AP

Synonyms

Ankycorbin, KIAA1334, NORPEG, RAI13, RAI14, retinoic acid induced 14

---

Validation Data Gallery

• 

  WB analysis of HeLa using 17507-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17507-1-AP (RAI14 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 17507-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 17507-1-AP (RAI14 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 17507-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 17507-1-AP (RAI14 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse testis using 17507-1-AP

  IP result of anti-RAI14 (IP:17507-1-AP, 5ug; Detection:17507-1-AP 1:1000) with mouse testis tissue lysate 4000ug.

• 

  IF Staining of HeLa using 17507-1-AP

  Immunofluorescent analysis of Hela cells, using RAI14 antibody 17507-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

---

Tested Applications

• Positive WB detected in: HeLa cells, human placenta tissue, mouse testis tissue
• Positive IP detected in: mouse testis tissue
• Positive IF detected in: Hela cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 5 publications below
• WB: See 9 publications below
• IHC: See 1 publications below
• IF: See 3 publications below
• IP: See 2 publications below

Product Information

17507-1-AP targets RAI14 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RAI14 fusion protein Ag11708
• Full Name: retinoic acid induced 14
• Calculated Molecular Weight: 980 aa, 110 kDa
• Observed Molecular Weight: 110 kDa
• GenBank Accession Number: BC052988
• Gene Symbol: RAI14
• Gene ID (NCBI): 26064
• RRID: AB_2175992
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
	WB	Genes Dev; Localization of Kif1c mRNA to cell protrusions dictates binding partner specificity of the encoded protein; Authors - Megan L Norris
human,mouse	WB,IP	Elife; Retinoic acid-induced protein 14 controls dendritic spine dynamics associated with depressive-like behaviors.; Authors - Soo Jeong Kim
mouse	WB,IF	Cells; Cysteine-Rich LIM-Only Protein 4 (CRP4) Promotes Atherogenesis in the ApoE-/- Mouse Model.; Authors - Natalie Längst
human	WB	Cell Transplant; RAI14 Regulated by circNFATC3/miR-23b-3p axis Facilitates Cell Growth and Invasion in Gastric Cancer.; Authors - XinXin Yan
rat	WB,IF,IP	PLoS One; Rai14 (retinoic acid induced protein 14) is involved in regulating f-actin dynamics at the ectoplasmic specialization in the rat testis*.; Authors - Xiaojing Qian ; KD Validated
mouse	WB,IF	PeerJ; Retinoic Acid Induced Protein 14 (Rai14) is dispensable for mouse spermatogenesis.; Authors - Yangyang Wu ; KO Validated