RAD17 Monoclonal antibody

RAD17 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1F12D8

Cat no : 68539-1-Ig

Synonyms

CCYC, FLJ41520, HRAD17, R24L, RAD17, RAD17 homolog (S. pombe), RAD17SP, RAD24, RF C/activator 1 homolog

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Validation Data Gallery

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  WB analysis using 68539-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68539-1-Ig (RAD17 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: LNCaP cells, HeLa cells, HEK-293 cells, Jurkat cells, Raji cells, Daudi cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68539-1-Ig targets RAD17 in WB, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: RAD17 fusion protein Ag4348
• Full Name: RAD17 homolog (S. pombe)
• Calculated Molecular Weight: 681 aa, 71 kDa
• Observed Molecular Weight: 70-80 kDa
• GenBank Accession Number: BC032304
• Gene Symbol: RAD17
• Gene ID (NCBI): 5884
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Rad17, a component of the checkpoint clamp loader complex (RAD17/RFC), was originally initially identified as a DNA damage checkpoint protein. Rad17 participates in cell cycle control and DNA damage repair. Rad17 phosphorylation by ATR (ataxia telangiectasia mutated and Rad3-related) kinase is indispensable for the recruitment of Claspin and the activation of Chk1 in response to genotoxic stress.