RABGAP1L Polyclonal antibody
RABGAP1L Polyclonal Antibody for WB, IP, IF, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, IF, IHC, ELISA
Conjugate
Unconjugated
Cat no : 13894-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HeLa cells, MCF-7 cells |
Positive IP detected in | HeLa cells |
Positive IHC detected in | human hepatocirrhosis tissue, human placenta tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | Hela cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Immunofluorescence (IF) | IF : 1:10-1:100 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
KD/KO | See 1 publications below |
WB | See 2 publications below |
IHC | See 1 publications below |
IF | See 1 publications below |
Product Information
13894-1-AP targets RABGAP1L in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | RABGAP1L fusion protein Ag4856 |
Full Name | RAB GTPase activating protein 1-like |
Calculated Molecular Weight | 815 aa, 93 kDa |
Observed Molecular Weight | 93-105 kDa |
GenBank Accession Number | BC041888 |
Gene Symbol | RABGAP1L |
Gene ID (NCBI) | 9910 |
RRID | AB_10638308 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
RABGAP1L, also known as TBC1D18, is a member of the Tre2/Bub2/Cdc16 (TBC)-domain-containing protein family. Overexpression of RABGAP1L potentiated the antiviral action of IFN against a panel of viruses, including IAV, and its function required a catalytically active TBC domain.
Protocols
Product Specific Protocols | |
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WB protocol for RABGAP1L antibody 13894-1-AP | Download protocol |
IHC protocol for RABGAP1L antibody 13894-1-AP | Download protocol |
IF protocol for RABGAP1L antibody 13894-1-AP | Download protocol |
IP protocol for RABGAP1L antibody 13894-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Nat Commun The oncogene AAMDC links PI3K-AKT-mTOR signaling with metabolic reprograming in estrogen receptor-positive breast cancer. | ||
Cell Rep Restriction factor screening identifies RABGAP1L-mediated disruption of endocytosis as a host antiviral defense. | ||
J Cell Biol TBC1D18 is a Rab5-GAP that coordinates endosome maturation together with Mon1
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