RAB9B Polyclonal antibody

RAB9B Polyclonal Antibody for WB, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 18152-1-AP

Synonyms

Rab 9 like protein, Rab 9L, RAB9B, RAB9L, Ras related protein Rab 9B

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Validation Data Gallery

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  WB analysis of HepG2 using 18152-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 18152-1-AP (RAB9B antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 18152-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 18152-1-AP (RAB9B antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 18152-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 18152-1-AP (RAB9B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 18152-1-AP

  IF result of RAB9B antibody (18152-1-AP, 1:200) detected with PFA fixed HeLa cells. Greem: RAB9B antibody stains late endosomes. Red, Golgi stain.

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  IF Staining of HepG2 using 18152-1-AP

  Immunofluorescent analysis of HepG2 cells, using RAB9B antibody 18152-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

• Positive WB detected in: HeLa cells, mouse brain tissue, HepG2 cells
• Positive IF detected in: HeLa cells, HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

18152-1-AP targets RAB9B in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RAB9B fusion protein Ag12848
• Full Name: RAB9B, member RAS oncogene family
• Calculated Molecular Weight: 201 aa, 23 kDa
• Observed Molecular Weight: 23-25 kDa
• GenBank Accession Number: BC093756
• Gene Symbol: RAB9B
• Gene ID (NCBI): 51209
• RRID: AB_10642146
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.