RAB39 Polyclonal antibody

• Featured Product
• KD/KO Validated

RAB39 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 13355-1-AP

Synonyms

Rab 39, RAB39, RAB39A, Ras related protein Rab 39A

---

Validation Data Gallery

• 

  WB analysis of mouse brain using 13355-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 13355-1-AP (RAB39 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 13355-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 13355-1-AP (RAB39 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 13355-1-AP

  IP result of anti-RAB39 (IP:13355-1-AP, 4ug; Detection:13355-1-AP 1:500) with mouse brain tissue lysate 4000ug.

• 

  IHC staining of human gliomas using 13355-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 13355-1-AP (RAB39 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 13355-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 13355-1-AP (RAB39 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 13355-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 13355-1-AP (RAB39 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 13355-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 13355-1-AP (RAB39 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human gliomas using 13355-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 13355-1-AP (RAB39 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human gliomas using 13355-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 13355-1-AP (RAB39 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human brain using 13355-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 13355-1-AP (RAB39 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human brain using 13355-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 13355-1-AP (RAB39 Antibody) at dilution of 1:200 (under 40x lens).

---

Tested Applications

• Positive WB detected in: mouse brain tissue, human brain tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human gliomas tissue, human brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Product Information

13355-1-AP targets RAB39 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, canine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RAB39 fusion protein Ag4151
• Full Name: RAB39, member RAS oncogene family
• Calculated Molecular Weight: 217 aa, 25 kDa
• Observed Molecular Weight: 25 kDa
• GenBank Accession Number: BC028064
• Gene Symbol: RAB39
• Gene ID (NCBI): 54734
• RRID: AB_2284908
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Nat Commun; C9orf72-catalyzed GTP loading of Rab39A enables HOPS-mediated membrane tethering and fusion in mammalian autophagy; Authors - Shen Zhang ; KD Validated
human	WB	Oncotarget; Prominent role of RAB39A-RXRB axis in cancer development and stemness.; Authors - Tokuhiro Chano
human	IF	J Biochem; RAB39A: a Rab small GTPase with a prominent role in cancer stemness.; Authors - Tokuhiro Chano
canine	WB,IP	Cell Rep; Rab39 and its effector UACA regulate basolateral exosome release from polarized epithelial cells.; Authors - Takahide Matsui