RAB38 Monoclonal antibody

RAB38 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

3A10C5

Cat no : 68603-1-Ig

Synonyms

Melanoma antigen NY MEL 1, NY MEL 1, RAB38, Ras related protein Rab 38, rrGTPbp

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Validation Data Gallery

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  WB analysis using 68603-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68603-1-Ig (RAB38 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of Mo7e using 68603-1-Ig

  Mo7e cells were subjected to SDS PAGE followed by western blot with 68603-1-Ig (RAB38 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: MCF-10A cells, Mo7e cells, HaCaT cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68603-1-Ig targets RAB38 in WB, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: RAB38 fusion protein Ag26440
• Full Name: RAB38, member RAS oncogene family
• Calculated Molecular Weight: 211 aa, 24 kDa
• Observed Molecular Weight: 24 kDa
• GenBank Accession Number: BC015808
• Gene Symbol: RAB38
• Gene ID (NCBI): 23682
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The RAB family of small GTPases is part of the Ras protein superfamily and is primarily responsible for regulating intracellular vesicle transport and migration. RAB38 is a recently identified member of the RAB family of proteins and its expression is tissue-specific. RAB38 is predominantly expressed in melanocytes and melanoma tissues, but not in other normal tissues, serving as a melanocyte differentiation antigen. RAB38 has also been reported to be upregulated in glioblastoma, and high levels of its expression are associated with a poor prognosis and enhanced cell migration.