RAB38 Polyclonal antibody

RAB38 Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 12234-1-AP

Synonyms

Melanoma antigen NY MEL 1, NY MEL 1, RAB38, Ras related protein Rab 38, rrGTPbp

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Validation Data Gallery

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  WB analysis of NIH/3T3 using 12234-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 12234-1-AP (RAB38 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of A375 using 12234-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 12234-1-AP (RAB38 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 12234-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 12234-1-AP (RAB38 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human gliomas using 12234-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12234-1-AP (RAB38 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 12234-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12234-1-AP (RAB38 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung using 12234-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 12234-1-AP (RAB38 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung using 12234-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 12234-1-AP (RAB38 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of A549 using 12234-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed A549 cells using 12234-1-AP (RAB38 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: NIH/3T3 cells, A375 cells
• Positive IHC detected in: human gliomas tissue, human lung tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: A549 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IF: See 1 publications below

Product Information

12234-1-AP targets RAB38 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RAB38 fusion protein Ag2874
• Full Name: RAB38, member RAS oncogene family
• Calculated Molecular Weight: 211 aa, 24 kDa
• Observed Molecular Weight: 24 kDa
• GenBank Accession Number: BC015808
• Gene Symbol: RAB38
• Gene ID (NCBI): 23682
• RRID: AB_2877839
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	EMBO Rep; Rab22A recruits BLOC-1 and BLOC-2 to promote the biogenesis of recycling endosomes.; Authors - Saurabh Shakya
mouse	IF	JBMR Plus; Interspecies Single-Cell RNA-Seq Analysis Reveals the Novel Trajectory of Osteoclast Differentiation and Therapeutic Targets; Authors - Yasunori Omata