RAB24 Polyclonal antibody

RAB24 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11445-1-AP

Synonyms

RAB24, Ras related protein Rab 24



Tested Applications

Positive WB detected inHEK-293 cells, human skeletal muscle tissue, fetal human brain tissue, mouse brain tissue, rat brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11445-1-AP targets RAB24 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, canine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RAB24 fusion protein Ag1995
Full Name RAB24, member RAS oncogene family
Calculated Molecular Weight 203 aa, 23 kDa
Observed Molecular Weight 23 kDa
GenBank Accession NumberBC021263
Gene Symbol RAB24
Gene ID (NCBI) 53917
RRIDAB_2269046
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RAB24 is a small GTPase of the Rab subfamily of Ras-related proteins that regulate intracellular protein trafficking. Unlike other Rab family members, RAB24 does not interact with GDP dissociation inhibitors (GDIs), including ARHGDIA and ARHGDIB. It Interacts with ZFYVE20. Rab24 modulates several mitotic events, including chromosome segregation and cytokinesis, perhaps through the interaction with microtubules.

Protocols

Product Specific Protocols
WB protocol for RAB24 antibody 11445-1-APDownload protocol
IHC protocol for RAB24 antibody 11445-1-APDownload protocol
IP protocol for RAB24 antibody 11445-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
canineIHC

PLoS Genet

Canine Hereditary Ataxia in Old English Sheepdogs and Gordon Setters Is Associated with a Defect in the Autophagy Gene Encoding RAB24.

Authors - Caryline Agler
humanWB

Dose Response

Genome-Wide Identification of Autophagy Prognostic Signature in Pancreatic Cancer.

Authors - Jianfa Yu