Phospho-PDH E1 Alpha (Ser300) Polyclonal antibody

Phospho-PDH E1 Alpha (Ser300) Polyclonal Antibody for WB, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human and More (1)

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 29583-1-AP

Synonyms

PDHA, PDHA1, PDHCE1A, PDHE1 A type I, PHE1A, Phospho-PDH E1 Alpha (Ser300)

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Validation Data Gallery

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  WB analysis using 29583-1-AP

  10 % fetal bovine serum treated HeLa cells and Serum-free treated HeLa cells were subjected to SDS PAGE followed by western blot with 29583-1-AP (Phospho-PDH E1 Alpha (Ser300) antibody) at dilution of 1:7000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

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  IF Staining of HepG2 using 29583-1-AP

  Immunofluorescent analysis of (4% PFA) fixed λ phosphatase treated HepG2 cells using Phospho-PDH E1 Alpha (Ser300) antibody (29583-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Tested Applications

• Positive WB detected in: Serum-free treated HeLa cells, 10 % fetal bovine serum treated HeLa cells
• Positive IF detected in: λ phosphatase treated HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:14000
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

29583-1-AP targets Phospho-PDH E1 Alpha (Ser300) in WB, IF, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: pyruvate dehydrogenase (lipoamide) alpha 1
• Calculated Molecular Weight: 43 kDa
• Observed Molecular Weight: 40 kDa
• GenBank Accession Number: BC002406
• Gene Symbol: PDHA1
• Gene ID (NCBI): 5160
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PDH E1 Alpha (PDHA1), as the major component of PDH, can be phosphorylated and inactivated by PDHK1. PDHA1 contains three serine residues (Ser232, Ser293, or Ser300) that can be reversibly phosphorylated by a dedicated family of four inhibitory pyruvate dehydrogenase kinases (PDHK1-4) and two reactivating phosphatases (PDP1, 2). Hypoxia induces the expression of PDHK1 and PDHK3 and hyperphosphorylates PDHA1. Phosphorylated PDHA1 inactivates the whole pyruvate dehydrogenase complex (PDC), reduces pyruvate entering into the tricarboxylic acid (TCA) cycle for oxidative phosphorylation, enhances the Warburg effect and promotes tumorigenesis. (PMID: 30993888, PMID: 34749809)

Publications

Species	Application	Title
mouse	WB	Cell Death Dis; Pyruvate dehydrogenase kinase 1 protects against neuronal injury and memory loss in mouse models of diabetes; Authors - Yuan Yao