Phospho-MYL9 (Thr19/Ser20) Recombinant antibody

Phospho-MYL9 (Thr19/Ser20) Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1G10

Cat no : 82717-1-RR

Synonyms

20 kDa myosin light chain, LC20, MLC 2C, MLC2, MRLC1, MYL9, Myosin RLC, MYRL2

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Validation Data Gallery

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  WB analysis using 82717-1-RR

  Non-treated and Calyculin A treated HeLa cells were subjected to SDS PAGE followed by western blot with 82717-1-RR (Phospho-MYL9 (Thr19/Ser20) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Actin antibody as loading control.

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  WB analysis using 82717-1-RR

  Non-treated and Calyculin A treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 82717-1-RR (Phospho-MYL9 (Thr19/Ser20) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Actin antibody as loading control.

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Tested Applications

• Positive WB detected in: Calyculin A treated NIH/3T3 cells, Calyculin A treated HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

82717-1-RR targets Phospho-MYL9 (Thr19/Ser20) in WB, ELISA applications and shows reactivity with Human, mouse samples.

• Tested Reactivity: Human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Peptide
• Full Name: myosin, light chain 9, regulatory
• Calculated Molecular Weight: 20 kDa
• Observed Molecular Weight: 19-20 kDa
• GenBank Accession Number: BC002648
• Gene Symbol: MYL9
• Gene ID (NCBI): 10398
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Myosin regulatory light polypeptide 9 (MYL9), also known as MLC2, belongs to the myosin regulatory subunits. It plays an important role in regulation of both smooth muscle and nonmuscle cell contractile activity via its phosphorylation at Thr19 and Ser20. Implicated in cytokinesis, receptor capping, and cell locomotion (PMID:11942626, PMID:2526655). Some studies have demonstrated that MYL9 may play important roles in various human cancers. The expression and phosphorylation of MYL9 (Thr19/Ser20) may be increased in human breast (PMID: 22144583) and liver cancers (PMID: 18648664), while decreased in human colon (PMID: 22752057) and bladder cancers (PMID: 21139803). MYL9 was the only gene differentially expressed in the aged versus young injured arteries in the rat smooth muscle cell layers (PMID:22003410).