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PE-conjugated Phospho-CREB1 (Ser133) Recombinant antibody

Phospho-CREB1 (Ser133) Recombinant Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

FC (Intra)

Conjugate

PE Fluorescent Dye

CloneNo.

5H5

Cat no : PE-81871

Synonyms

CREB, CREB 1, CREB1

Validation Data Gallery

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  • Flow cytometry (FC) experiment of HEK-293 cells using PE-conjugated Phospho-CREB1 (Ser133) Recombinant a (PE-81871)

    FC experiment of HEK-293 using PE-81871

    1X10^6 HEK-293 cells untreated (dashed lines) or treated with Calyculin A (red) were intracellularly stained with 0.125 ug PE Anti-Human Phospho-CREB1 (Ser133) (PE-81871, Clone:5H5), or 0.125 ug Control Antibody (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH.

Tested Applications

Positive FC detected inCalyculin A treated HEK-293 cells

Recommended dilution

ApplicationDilution
Flow Cytometry (FC)FC : 0.13 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

PE-81871 targets Phospho-CREB1 (Ser133) in FC (Intra) applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide
Full Name cAMP responsive element binding protein 1
Calculated Molecular Weight 341 aa, 35 kDa
Observed Molecular Weight 35-46 kDa
GenBank Accession NumberBC010636
Gene Symbol CREB1
Gene ID (NCBI) 1385
Conjugate PE Fluorescent Dye
Excitation/Emission Maxima Wavelengths496 nm, 565 nm / 578 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.09% sodium azide and 0.5% BSA.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment. 20ul sizes contain 0.1% BSA.

Background Information

The cAMP-response element binding protein (CREB) is localized in the nucleus and acts as a transcription factor, which binds to the cAMP response element (CRE) of the promoters of its target genes, upon phosphorylation at Ser133 by different receptor-activated protein kinases, such as protein kinase A (PKA), calmodulin-dependent protein kinase (CaMK), mitogen-activated protein kinases (MAPK), and other kinases. The activity of CREB1 in neurons has been correlated with various intracellular processes, including proliferation, differentiation, survival, long-term synaptic potentiation, neurogenesis, and neuronal plasticity. CREB1 belongs to the CREB/activating transcription factor (ATF) family of transcription factors. The antibody also detects the phosphorylated form of the CREB-related protein ATF1. (PMID: 30214393, PMID: 33917483, PMID: 32605164)