CoraLite® Plus 488-conjugated PYCRL Monoclonal antibody

PYCRL Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1G7A3

Cat no : CL488-68087

Synonyms

FLJ13852, PYCRL

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Validation Data Gallery

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  FC experiment of HEK-293T using CL488-68087

  1X10^6 HEK-293T cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human PYCRL (CL488-68087, Clone:1G7A3) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive FC detected in: HEK-293T cells

Recommended dilution

• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-68087 targets PYCRL in FC (Intra) applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: PYCRL fusion protein Ag31938
• Full Name: pyrroline-5-carboxylate reductase-like
• Calculated Molecular Weight: 274 aa, 29 kDa
• Observed Molecular Weight: 26-29 kDa
• GenBank Accession Number: BC007993
• Gene Symbol: PYCRL
• Gene ID (NCBI): 65263
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.