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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated PYCR1 Polyclonal antibody

PYCR1 Polyclonal Antibody for FC (Intra), IF

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-13108

Synonyms

P5C, P5C reductase 1, P5CR, P5CR 1, PIG45, PP222, PYCR, PYCR1

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite® Plus 488-conjugated PYCR1 Polyclonal ant (CL488-13108)

    IF Staining of HepG2 using CL488-13108

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 PYCR1 antibody (CL488-13108) at dilution of 1:200.

  • Flow cytometry (FC) experiment of HepG2 cells using CoraLite® Plus 488-conjugated PYCR1 Polyclonal ant (CL488-13108)

    FC experiment of HepG2 using CL488-13108

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human PYCR1 (CL488-13108) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive IF detected inHepG2 cells
Positive FC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-13108 targets PYCR1 in IF, FC (Intra) applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PYCR1 fusion protein Ag3764
Full Name pyrroline-5-carboxylate reductase 1
Calculated Molecular Weight 319 aa, 33.8 kDa
Observed Molecular Weight 33 kDa, 35 kDa
GenBank Accession NumberBC022244
Gene Symbol PYCR1
Gene ID (NCBI) 5831
RRIDAB_2919068
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PYCR1,also named as P5CR1, belongs to the pyrroline-5-carboxylate reductase family. It is a housekeeping enzyme that catalyzes the last step in proline biosynthesis. PYCR1 can utilize both NAD and NADP, but has higher affinity for NAD. It is involved in the cellular response to oxidative stress. Mutation in PYCR1 will cause ARCL type II(ARCL2B ). Some mutation will cause DeBarsy syndrome (DBS) which is characterized by progeroid features, ophthalmological abnormalities, intrauterine growth retardation, and cutis laxa. The MW of PYCR1 is about 33-35 kDa. PYCR1 has 3 isoforms produced by alternative splicing. This antibody may have cross reaction to PYCR2 due to the high homology.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 PYCR1 antibody CL488-13108Download protocol
FC protocol for CL Plus 488 PYCR1 antibody CL488-13108Download protocol
Standard Protocols
Click here to view our Standard Protocols