PXR Monoclonal antibody, PBS Only

PXR Monoclonal Antibody for WB, IHC, FC (Intra), Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1G3A7

Cat no : 67912-1-PBS

Synonyms

BXR, NR1I2, ONR1, Orphan nuclear receptor PAR1, Orphan nuclear receptor PXR, PAR, PAR1, PAR2, PARq, Pregnane X receptor, PRR, PXR, SAR, SXR

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Validation Data Gallery

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  WB analysis using 67912-1-Ig (same clone as 67912-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67912-1-Ig (PXR antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67912-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 67912-1-Ig (same clone as 67912-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 67912-1-Ig (PXR antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67912-1-PBS in a different storage buffer formulation.

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  WB analysis of COLO 320 using 67912-1-Ig (same clone as 67912-1-PBS)

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 67912-1-Ig (PXR antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67912-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 67912-1-Ig (same clone as 67912-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 67912-1-Ig (PXR antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67912-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 67912-1-Ig (same clone as 67912-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 67912-1-Ig (PXR antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67912-1-PBS in a different storage buffer formulation.

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  FC experiment of MCF-7 using 67912-1-Ig (same clone as 67912-1-PBS)

  1X10^6 MCF-7 cells were intracellularly stained with 0.2 ug Anti-Human PXR (67912-1-Ig, Clone:1G3A7) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 67912-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67912-1-PBS targets PXR in WB, IHC, FC (Intra), Indirect ELISA applications and shows reactivity with Human, Mouse, Rat samples.

• Tested Reactivity: Human, Mouse, Rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: PXR fusion protein Ag7991
• Full Name: nuclear receptor subfamily 1, group I, member 2
• Calculated Molecular Weight: 50 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: BC017304
• Gene Symbol: NR1I2
• Gene ID (NCBI): 8856
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Pregnane X receptor (PXR; NR1I2), a member of the nuclear receptor superfamily, plays a key role in the induction of genes involved in drug transport and metabolism. It modulates drug transport and metabolism through the regulation of target genes responsible for the transport and conversion of chemicals into metabolites that are more easily eliminated from the body [PMID: 22609277]. It is also emerging as an endobiotic receptor that regulates the inflammatory response. In addition, PXR acts as a transcription factor that activates the transcription of multiple genes involved in the metabolism and secretion of potentially harmful xenobiotics, drugs and endogenous compounds [PMID: 19297428].