PSME1 Monoclonal antibody

PSME1 Monoclonal Antibody for WB, IF, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IF, ELISA

Conjugate

Unconjugated

CloneNo.

1F7E2

Cat no : 67928-1-Ig

Synonyms

IFI5111, IGUP I 5111, PA28A, PA28alpha, PSME1, REG alpha, REGalpha

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Validation Data Gallery

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  WB analysis using 67928-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67928-1-Ig (PSME1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

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  IF Staining of MCF-7 using 67928-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using PSME1 antibody (67928-1-Ig, Clone: 1F7E2 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: MDA-MB-231 cells, Hela cells, HEK-293 cells, Jurkat cells, k-562 cells, HSC-T6 cells, C6 cells, NIH/3T3 cells and 4T1 cells.
• Positive IF detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF): IF : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

67928-1-Ig targets PSME1 in WB, IF, ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: PSME1 fusion protein Ag30549
• Full Name: proteasome (prosome, macropain) activator subunit 1 (PA28 alpha)
• Calculated Molecular Weight: 29 kDa
• Observed Molecular Weight: 29 kDa
• GenBank Accession Number: BC007503
• Gene Symbol: PSME1
• Gene ID (NCBI): 5720
• RRID: AB_2918680
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The principal function of the proteasome is targeted degradation of intracellular proteins. Activity of the 20S proteasome is controlled by regulatory complexes that bind to the ends of the cylindrical proteasome. 11S regulator (REG or PA28), is a complex of 28 kDa subunits that is thought to activate proteasomes toward the production of antigenic peptides. Human PSME1 and PSME2 genes encode the two proteasome activators PA28 alpha and beta, respectively, which have been implicated in antigen processing for loading class I MHC molecules. The PA28 activator complex enhances the generation of class I binding peptides by altering the cleavage pattern of the proteasome.