PSMD5 Monoclonal antibody

PSMD5 Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG3

Reactivity

Human, pig, rat, rabbit, mouse

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

1D5F7

Cat no : 67682-1-Ig

Synonyms

26S protease subunit S5 basic, 26S proteasome subunit S5B, KIAA0072, PSMD5, S5B

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Validation Data Gallery

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  WB analysis of pig liver using 67682-1-Ig

  pig liver tissue were subjected to SDS PAGE followed by western blot with 67682-1-Ig (PSMD5 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 67682-1-Ig

  K-562 cells were subjected to SDS PAGE followed by western blot with 67682-1-Ig (PSMD5 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HCT 116 using 67682-1-Ig

  HCT 116 cells were subjected to SDS PAGE followed by western blot with 67682-1-Ig (PSMD5 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rabbit liver using 67682-1-Ig

  rabbit liver tissue were subjected to SDS PAGE followed by western blot with 67682-1-Ig (PSMD5 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat liver using 67682-1-Ig

  rat liver tissue were subjected to SDS PAGE followed by western blot with 67682-1-Ig (PSMD5 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon cancer using 67682-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67682-1-Ig (PSMD5 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 67682-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67682-1-Ig (PSMD5 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of A431 using 67682-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using PSMD5 antibody (67682-1-Ig, Clone: 1D5F7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red).

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Tested Applications

• Positive WB detected in: pig liver tissue, rabbit liver tissue, rat liver tissue, K-562 cells, HCT 116 cells
• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

67682-1-Ig targets PSMD5 in WB, IHC, IF, ELISA applications and shows reactivity with Human, pig, rat, rabbit, mouse samples.

• Tested Reactivity: Human, pig, rat, rabbit, mouse
• Host / Isotype: Mouse / IgG3
• Class: Monoclonal
• Type: Antibody
• Immunogen: PSMD5 fusion protein Ag19298
• Full Name: proteasome (prosome, macropain) 26S subunit, non-ATPase, 5
• Calculated Molecular Weight: 504 aa, 56 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: BC014478
• Gene Symbol: PSMD5
• Gene ID (NCBI): 5711
• RRID: AB_2882875
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.