PSMD3 Polyclonal antibody

PSMD3 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 12054-1-AP

Synonyms

P58, Proteasome subunit p58, PSMD3, RPN3, S3

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Validation Data Gallery

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  WB analysis using 12054-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12054-1-AP (PSMD3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 12054-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12054-1-AP (PSMD3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 12054-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12054-1-AP (PSMD3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human kidney using 12054-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 12054-1-AP (PSMD3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse kidney using 12054-1-AP

  IP result of anti-PSMD3 (IP:12054-1-AP, 4ug; Detection:12054-1-AP 1:500) with mouse kidney tissue lysate 4000ug.

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  IHC staining of human colon cancer using 12054-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12054-1-AP (PSMD3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human small intestine using 12054-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 12054-1-AP (PSMD3 antibody) at dilution of 1:500 (under 10x lens).

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  IHC staining of human small intestine using 12054-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 12054-1-AP (PSMD3 antibody) at dilution of 1:500 (under 40x lens).

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  IHC staining of human breast cancer using 12054-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12054-1-AP (PSMD3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 12054-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12054-1-AP (PSMD3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 12054-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12054-1-AP (PSMD3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: A549 cells, mouse brain tissue, human kidney tissue, human brain tissue, HEK-293 cells, mouse kidney tissue, rat kidney tissue
• Positive IP detected in: mouse kidney tissue
• Positive IHC detected in: human colon cancer tissue, human breast cancer tissue, human small intestine tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below
• IF: See 1 publications below

Product Information

12054-1-AP targets PSMD3 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PSMD3 fusion protein Ag2684
• Full Name: proteasome (prosome, macropain) 26S subunit, non-ATPase, 3
• Calculated Molecular Weight: 534 aa, 61 kDa
• Observed Molecular Weight: 61 kDa
• GenBank Accession Number: BC012302
• Gene Symbol: PSMD3
• Gene ID (NCBI): 5709
• RRID: AB_2170484
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PSMD3, encoding proteasome (prosome, macropain) 26S subunit, non-ATPase 3, a member of the 26S proteasome family. PSMD3 is expressed in most of the tissues according to the RNA-Seq database. It has been reported that PSMD3 genotypes are associated with white blood cell (WBC) and neutrophil counts and have interactions with dietary fatty acids and carbohydrates on glucose-related traits. PSMD3 is located at ch17q21 and encodes a protein with a molecular weight of 61 kDa (PMID: 31013812).

Publications

Species	Application	Title
human	WB	Sci Rep; Insights into the ubiquitin-proteasome system of human embryonic stem cells.; Authors - Isabel Saez
human	WB	Protein Cell; DSSylation, a novel protein modification targets proteins induced by oxidative stress, and facilitates their degradation in cells.; Authors - Yinghao Zhang
human	WB	Cell Death Dis; ALG-2 couples T cell activation and apoptosis by regulating proteasome activity and influencing MCL1 stability.; Authors - Tian-Sheng He
human	WB,IF	Cell Mol Life Sci; The contribution of proteasomal impairment to autophagy activation by C9orf72 poly-GA aggregates; Authors - Mei Pu