CoraLite®594-conjugated PSMB8 Monoclonal antibody

PSMB8 Monoclonal Antibody for IF, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse

Applications

IF, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

2A5B6

Cat no : CL594-66759

Synonyms

beta5i, D6S216, D6S216E, LMP7, Low molecular mass protein 7, Macropain subunit C13, Proteasome component C13, Proteasome subunit beta 5i, Proteasome subunit beta type 8, PSMB5i, PSMB8, RING10, Y2

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Validation Data Gallery

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  IF Staining of C2C12 using CL594-66759

  N/A

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  IF Staining of C2C12 using CL594-66759

  Immunofluorescent analysis of (4% PFA) fixed C2C12 cells using CoraLite®594 PSMB8 antibody (CL594-66759, Clone: 2A5B6 ) at dilution of 1:200, CL488-phalloidin (blue).

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  IF Staining of human liver cancer using CL594-66759

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using CoraLite®594 PSMB8 antibody (CL594-66759, Clone: 2A5B6 ) at dilution of 1:200.

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  IF Staining of human liver cancer using CL594-66759

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using CoraLite®594 PSMB8 antibody (CL594-66759, Clone: 2A5B6 ) at dilution of 1:200.

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  IF Staining of HeLa using CL594-66759

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite®594 PSMB8 antibody (CL594-66759, Clone: 2A5B6 ) at dilution of 1:200.

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  FC experiment of HeLa using CL594-66759

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human PSMB8 (CL594-66759, Clone:2A5B6) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

• Positive IF detected in: C2C12 cells, human liver cancer tissue, HeLa cells
• Positive FC detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-66759 targets PSMB8 in IF, FC (Intra) applications and shows reactivity with Human, mouse samples.

• Tested Reactivity: Human, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: PSMB8 fusion protein Ag6780
• Full Name: proteasome (prosome, macropain) subunit, beta type, 8 (large multifunctional peptidase 7)
• Calculated Molecular Weight: 30 kDa
• Observed Molecular Weight: 23 kDa
• GenBank Accession Number: BC001114
• Gene Symbol: PSMB8
• Gene ID (NCBI): 5696
• RRID: AB_2920031
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PSMB8(Proteasome subunit beta type-8) is also named as LMP7, PSMB5i, RING10, Y2 and belongs to the peptidase T1B family. The gene encodes the chymotrypsin-like catalytic subunit of the immunoproteasome(PMID: 19525961). PSMB8 has a role in controlling pathogenic immune responses and may be a target in autoimmune disorders. Its prosequence is not essential for incorporation of PSMB8 into the maturing proteasome, but it increased the efficiency of PSMB8 incorporation and proteasome maturation(PMID: 10926487). The pro-PSMB8 is a 276aa protein with the molecular mass of 30 kDa, and the mature form is about 23kDa due to the 72aa propeptide cleaved. Defects in PSMB8 are the cause of Nakajo syndrome (NKJO).