PSMB4 Polyclonal antibody

• Featured Product
• KD/KO Validated

PSMB4 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (2)

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

5

Cat no : 11029-1-AP

Synonyms

20S proteasome, 26 kDa prosomal protein, HN3, HsBPROS26, HsN3, Macropain beta chain, PROS 26, PROS26, Proteasome beta chain, Proteasome chain 3, Proteasome subunit beta type 4, PSMB4

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Validation Data Gallery

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  WB analysis using 11029-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11029-1-AP (PSMB4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 11029-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 11029-1-AP (PSMB4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of human skeletal muscle using 11029-1-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11029-1-AP (PSMB4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse skeletal muscle using 11029-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11029-1-AP (PSMB4 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 11029-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 11029-1-AP (PSMB4 antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of MCF-7 using 11029-1-AP

  Immunofluorescent analysis of MCF-7 cells, using PSMB4 antibody 11029-1-AP at 1:50 dilution and FITC-labeled donkey anti-rabbit IgG(green). Blue pseudocolor = DAPI (fluorescent DNA dye).

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  IF Staining of HepG2 using 11029-1-AP

  Immunofluorescent analysis of HepG2 cells using 11029-1-AP (PSMB4 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HEK-293 cells, mouse skeletal muscle tissue, human placenta tissue, human skeletal muscle tissue, MCF-7 cells
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: MCF-7 cells, HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 5 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

11029-1-AP targets PSMB4 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, monkey, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PSMB4 fusion protein Ag1500
• Full Name: proteasome (prosome, macropain) subunit, beta type, 4
• Calculated Molecular Weight: 29 kDa
• Observed Molecular Weight: 25 kDa
• GenBank Accession Number: BC010088
• Gene Symbol: PSMB4
• Gene ID (NCBI): 5692
• RRID: AB_2172040
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PSMB4(Proteasome subunit beta type-4) is also named as PROS26 and belongs to the peptidase T1B family. The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The human protein PSMB4 is known mainly as an interaction partner for several proteins, suggesting an important function of binding not only to the lid, but also to the 20 S core of the proteasome. PSMB4 might be a major site for proteasome regulation, where signals from the outside might be transduced to the protease activities inside(PMID:15660529 ). The full length 30 kDa protein has a propeptide of 45 amino acid.

Publications

Species	Application	Title
human	WB	Cancer Res; Comparative oncogenomics identifies PSMB4 and SHMT2 as potential cancer driver genes.; Authors - Genee Y Lee
mouse	WB	J Biol Chem; Hepatocyte-specific Wtap deficiency promotes hepatocellular carcinoma by activating GRB2/ERK depending on downregulation of proteasome-related genes; Authors - Xinzhi Li
human	WB,IHC	Oncol Rep; PSMB4 overexpression enhances the cell growth and viability of breast cancer cells leading to a poor prognosis.; Authors - Hua Wang ; KD Validated
human,pig,monkey	WB,IF	J Virol; PSMB4 Degrades the Porcine Reproductive and Respiratory Syndrome Virus Nsp1α Protein via the Autolysosome Pathway and Induces the Production of Type I Interferon; Authors - Heyou Yi ; KD Validated
mouse	WB	JHEP Rep; Downregulation of hepatic METTL3 contributes to APAP-induced liver injury in mice; Authors - Chunhong Liu