PSMA2 Polyclonal antibody

PSMA2 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 14377-1-AP

Synonyms

20S proteasome, HC3, Macropain subunit C3, MU, PMSA2, Proteasome component C3, PSC2, PSC3, PSMA2

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Validation Data Gallery

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  WB analysis of NIH/3T3 using 14377-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 14377-1-AP (PSMA2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 14377-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 14377-1-AP (PSMA2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 14377-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 14377-1-AP (PSMA2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 14377-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 14377-1-AP (PSMA2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 14377-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 14377-1-AP (PSMA2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 14377-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 14377-1-AP (PSMA2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 14377-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 14377-1-AP (PSMA2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 14377-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 14377-1-AP (PSMA2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human skeletal muscle using 14377-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 14377-1-AP (PSMA2 antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human skeletal muscle using 14377-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 14377-1-AP (PSMA2 antibody) at dilution of 1:200 (under 40x lens).

• 

  IF Staining of HepG2 using 14377-1-AP

  Immunofluorescent analysis of HepG2 cells using 14377-1-AP (PSMA2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: NIH/3T3 cells, HEK-293 cells, HepG2 cells, mouse kidney tissue, PC-3 cells
• Positive IHC detected in: human skeletal muscle tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 1 publications below

Product Information

14377-1-AP targets PSMA2 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PSMA2 fusion protein Ag5753
• Full Name: proteasome (prosome, macropain) subunit, alpha type, 2
• Calculated Molecular Weight: 26 kDa
• Observed Molecular Weight: 26 kDa
• GenBank Accession Number: BC047697
• Gene Symbol: PSMA2
• Gene ID (NCBI): 5683
• RRID: AB_2878050
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PSMA2 (Proteasome subunit alpha type-2) is the essential subunit that contributes to the complete assembly of 20S proteasome complex, involved in the proteolytic degradation of most intracellular proteins. It is required in spermatogenesis and generation of a subset of MHC class I-presented antigenic peptides(PMID: 34711951, PMID: 27342858). Dysregulation of PSMA2 degradation leads to tumorigenesis including cervical cancer (PMID:33684886).

Publications

Species	Application	Title
human	WB	Acta Biomater; Pushpin-like nanozyme for plasmon-enhanced tumor targeted therapy; Authors - Baofu Ma
human	IHC	J Ovarian Res; Development and clinical validation of a seven-gene signature based on tumor stem cell-related genes to predict ovarian cancer prognosis; Authors - Guangwei Wang