CoraLite®594-conjugated PSMA/GCPII Monoclonal antibody
PSMA/GCPII Monoclonal Antibody for IF, FC (Intra)
Host / Isotype
Mouse / IgG1
Reactivity
Human, Rat
Applications
IF, FC (Intra)
Conjugate
CoraLite®594 Fluorescent Dye
CloneNo.
3G4E12
Cat no : CL594-66678
Synonyms
Validation Data Gallery
Tested Applications
Positive IF detected in | PC-3 cells, human prostate cancer tissue |
Positive FC detected in | LNCaP cells |
Recommended dilution
Application | Dilution |
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Immunofluorescence (IF) | IF : 1:50-1:500 |
Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
CL594-66678 targets PSMA/GCPII in IF, FC (Intra) applications and shows reactivity with Human, Rat samples.
Tested Reactivity | Human, Rat |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | PSMA/GCPII fusion protein Ag16594 |
Full Name | folate hydrolase (prostate-specific membrane antigen) 1 |
Calculated Molecular Weight | 719 aa, 81 kDa |
Observed Molecular Weight | 100-120 kDa |
GenBank Accession Number | BC025672 |
Gene Symbol | FOLH1 |
Gene ID (NCBI) | 2346 |
RRID | AB_2920020 |
Conjugate | CoraLite®594 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 588 nm / 604 nm |
Form | Liquid |
Purification Method | Protein G purification |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
PSMA(Prostate-specific membrane antigen) is also named as FOLH1, FOLH, NAALAD1, PSM and belongs to the peptidase M28 family. PSMA is a 100-120 kDa integral transmembrane glycoprotein, considered to be a highly specific marker of the prostate gland, and has successfully been used as a marker of circulating prostatic epithelial cells(PMID:10074909; 15680901). It is involved in conversion of the major neurotransmitter (NAAG) to NAA and free glutamate. It has 8 isoforms produced by alternative splicing.
Protocols
Product Specific Protocols | |
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IF protocol for CL594 PSMA/GCPII antibody CL594-66678 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |