PRPF38A Polyclonal antibody

PRPF38A Polyclonal Antibody for WB, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 24946-1-AP

Synonyms

Pre mRNA splicing factor 38A, PRPF38A, RP5 965L7.1

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Validation Data Gallery

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  WB analysis of K-562 using 24946-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 24946-1-AP (PRPF38A Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 24946-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 24946-1-AP (PRPF38A Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HepG2 using 24946-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 24946-1-AP (PRPF38A antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: K-562 cells, HepG2 cells
• Positive IF detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 24946-1-AP is PRPF38A Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PRPF38A fusion protein Ag20980
• Full Name: PRP38 pre-mRNA processing factor 38 (yeast) domain containing A
• Calculated Molecular Weight: 312 aa, 37 kDa
• Observed Molecular Weight: 37-40 kDa
• GenBank Accession Number: BC000777
• Gene Symbol: PRPF38A
• Gene ID (NCBI): 84950
• RRID: AB_2879814
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.