PRM2 Polyclonal antibody

PRM2 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

10

Cat no : 14500-1-AP

Synonyms

P2, P2 Sperm histone HP3, PRM2, protamine 2, Sperm histone P2, Sperm protamine P2, Sperm protamine P3

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Validation Data Gallery

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  WB analysis of human testis using 14500-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 14500-1-AP (PRM2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human testis using 14500-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 14500-1-AP (PRM2 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human testis using 14500-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 14500-1-AP (PRM2 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: human testis tissue
• Positive IHC detected in: human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 6 publications below
• IHC: See 1 publications below
• IF: See 4 publications below

Product Information

14500-1-AP targets PRM2 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PRM2 fusion protein Ag5952
• Full Name: protamine 2
• Calculated Molecular Weight: 13 kDa
• Observed Molecular Weight: 10-25 kDa
• GenBank Accession Number: BC066338
• Gene Symbol: PRM2
• Gene ID (NCBI): 5620
• RRID: AB_2721024
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Mol Cell; Chromodomain Protein CDYL Acts as a Crotonyl-CoA Hydratase to Regulate Histone Crotonylation and Spermatogenesis.; Authors - Shumeng Liu
mouse	IF	J Hazard Mater; Multi-omics analyses reveal the mechanisms of Arsenic-induced male reproductive toxicity in mice.; Authors - Zijun Peng
mouse	WB	Environ Pollut; Silica nanoparticles induce spermatogenesis disorders via L3MBTL2-DNA damage-p53 apoptosis and RNF8-ubH2A/ubH2B pathway in mice.; Authors - Jianhui Liu
mouse	WB	Environ Pollut; Silica nanoparticles inhibiting the differentiation of round spermatid and chromatin remodeling of haploid period via MIWI in mice.; Authors - Jianhui Liu
human	WB	Hum Reprod; Lysine glutarylation in human sperm is associated with progressive motility.; Authors - Yi-Min Cheng
human		Hum Reprod; Posttranslational lysine 2-hydroxyisobutyrylation of human sperm tail proteins affects motility.; Authors - Yi-Min Cheng