PRKD3 Polyclonal antibody

PRKD3 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 12785-1-AP

Synonyms

EPK2, PKC NU, PKD3, PRKCN, PRKD3, Protein kinase C nu type, protein kinase D3, Protein kinase EPK2

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Validation Data Gallery

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  WB analysis using 12785-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12785-1-AP (PRKD3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 12785-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12785-1-AP (PRKD3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of DU 145 using 12785-1-AP

  DU 145 cells were subjected to SDS PAGE followed by western blot with 12785-1-AP (PRKD3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 12785-1-AP

  IP result of anti-PRKD3 (IP:12785-1-AP, 4ug; Detection:12785-1-AP 1:300) with HEK-293 cells lysate 2800ug.

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  IHC staining of human breast cancer using 12785-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12785-1-AP (PRKD3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 12785-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12785-1-AP (PRKD3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 12785-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12785-1-AP (PRKD3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 12785-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12785-1-AP (PRKD3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: DU 145 cells, HeLa cells, NIH/3T3 cells, C2C12 cells, Raji cells, Ramos cells
• Positive IP detected in: HEK-293 cells
• Positive IHC detected in: human breast cancer tissue, human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Product Information

12785-1-AP targets PRKD3 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PRKD3 fusion protein Ag3499
• Full Name: protein kinase D3
• Calculated Molecular Weight: 611aa,68 kDa; 100 kDa; 890aa
• Observed Molecular Weight: 68 kDa, 100 kDa
• GenBank Accession Number: BC030706
• Gene Symbol: PRKD3
• Gene ID (NCBI): 23683
• RRID: AB_2877881
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB,IF,IP	Exp Mol Med; Clusterin negatively modulates mechanical stress-mediated ligamentum flavum hypertrophy through TGF-β1 signaling; Authors - Chunlei Liu
human	WB	NAR Cancer; Estrogen receptor alpha mutations regulate gene expression and cell growth in breast cancer through microRNAs; Authors - Spencer Arnesen