PREX2 Polyclonal antibody

PREX2 Polyclonal Antibody for IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 24195-1-AP

Synonyms

DEP.2, DEPDC2, P REX2, PREX2

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Validation Data Gallery

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  IHC staining of human heart using 24195-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 24195-1-AP (PREX2 antibody) at dilution of 1:200 (under 10x lens)..

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  IHC staining of human heart using 24195-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 24195-1-AP (PREX2 antibody) at dilution of 1:200 (under 40x lens)..

• 

  IHC staining of human cerebellum using 24195-1-AP

  Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 24195-1-AP (PREX2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cerebellum using 24195-1-AP

  Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 24195-1-AP (PREX2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 24195-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 24195-1-AP (PREX2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 24195-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 24195-1-AP (PREX2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of U2OS using 24195-1-AP

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using 24195-1-AP (PREX2 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive IHC detected in: human heart tissue, human cerebellum tissue, human brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: U2OS cells

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 24195-1-AP is PREX2 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PREX2 fusion protein Ag19439
• Full Name: phosphatidylinositol-3,4,5-trisphosphate-dependent Rac exchange factor 2
• Calculated Molecular Weight: 1606 aa, 183 kDa
• Observed Molecular Weight: 182 kDa
• GenBank Accession Number: BC148795
• Gene Symbol: PREX2
• Gene ID (NCBI): 80243
• RRID: AB_2879450
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.