PPT1 Polyclonal antibody

• Featured Product
• KD/KO Validated

PPT1 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 29653-1-AP

Synonyms

CLN1, INCL, Palmitoyl protein hydrolase 1, PPT, PPT 1, PPT1

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Validation Data Gallery

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  WB analysis using 29653-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29653-1-AP (PPT1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 29653-1-AP

  WB result of PPT1 antibody (29653-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PPT1 transfected A549 cells.

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  WB analysis of A549 using 29653-1-AP

  WB result of PPT1 antibody (29653-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PPT1 transfected A549 cells.

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  IHC staining of human kidney using 29653-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 29653-1-AP (PPT1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 29653-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 29653-1-AP (PPT1 antibody) at dilution of 1:400 (under 10x lens). Heat-mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse testis using 29653-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using PPT1 antibody (29653-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HEK-293 cells, A549 cells, HeLa cells, SH-SY5Y cells, mouse testis tissue, rat testis tissue
• Positive IHC detected in: human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: mouse testis tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IF: See 1 publications below

Product Information

29653-1-AP targets PPT1 in WB, IF, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PPT1 fusion protein Ag31240
• Full Name: palmitoyl-protein thioesterase 1
• Calculated Molecular Weight: 34 kDa
• Observed Molecular Weight: 32-34 kDa
• GenBank Accession Number: BC008426
• Gene Symbol: PPT1
• Gene ID (NCBI): 5538
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The defective gene behind the INCL(infantile neuronal ceroid lipofuscinoses) disease, CLN1, encodes for palmitoyl protein thioesterase 1 (PPT1). It consists of 306 amino acids, including a signal sequence of 26 amino acids and three N-linked glycosylation sites. This protein exists with a molecular mass of 32 kDa, 34 kDa,36 kDa, and 38 kDa. The enzyme is transported into lysosomes of non-neuronal cells by the mannose 6-phosphate receptor (M6PR) mediated pathway and a significant amount of native PPT1 resides in a complex rather than in a monomeric form(PMID:17565660).

Publications

Species	Application	Title
mouse	WB,IF	Cell Rep Med; Targeting phenylpyruvate restrains excessive NLRP3 inflammasome activation and pathological inflammation in diabetic wound healing; Authors - Dongming Lv ; KD Validated