PPP6C Polyclonal antibody

PPP6C Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, CoIP, ELISA

Conjugate

Unconjugated

Publications

5

Cat no : 15852-1-AP

Synonyms

PP6C, PPP6, PPP6C

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Validation Data Gallery

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  WB analysis using 15852-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15852-1-AP (PPP6C antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 15852-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 15852-1-AP (PPP6C antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human testis using 15852-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 15852-1-AP (PPP6C antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 15852-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 15852-1-AP (PPP6C antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse testis using 15852-1-AP

  IP result of anti-PPP6C (IP:15852-1-AP, 3ug; Detection:15852-1-AP 1:500) with mouse testis tissue lysate 4000ug.

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  IHC staining of mouse testis using 15852-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 15852-1-AP (PPP6C antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 15852-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 15852-1-AP (PPP6C antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse testis tissue, HEK-293 cells, human testis tissue, NIH/3T3 cells, rat testis tissue
• Positive IP detected in: mouse testis tissue
• Positive IHC detected in: mouse testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 4 publications below
• IP: See 2 publications below
• CoIP: See 2 publications below

Product Information

15852-1-AP targets PPP6C in WB, IP, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, canine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PPP6C fusion protein Ag8670
• Full Name: protein phosphatase 6, catalytic subunit
• Calculated Molecular Weight: 305 aa, 35 kDa
• Observed Molecular Weight: 35 kDa
• GenBank Accession Number: BC006990
• Gene Symbol: PPP6C
• Gene ID (NCBI): 5537
• RRID: AB_2168894
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB,CoIP	Protein Cell; Dephosphorylation of cGAS by PPP6C impairs its substrate binding activity and innate antiviral response.; Authors - Mi Li
		J Biol Chem; Leucine Carboxyl Methyltransferase 1 (LCMT-1) Methylates Protein Phosphatase 4 (PP4) and Protein Phosphatase 6 (PP6) and Differentially Regulates the Stable Formation of Different PP4 Holoenzymes.; Authors - Juyeon Hwang
human	WB,IP	Cell Death Dis; PP6 negatively modulates LUBAC-mediated M1-ubiquitination of RIPK1 and c-FLIPL to promote TNFα-mediated cell death; Authors - Guowei Wu ; KO Validated
mouse,human	WB,IP,CoIP	Mol Cell; Energy sensor AMPK gamma regulates translation via phosphatase PPP6C independent of AMPK alpha; Authors - Qi Zhou
canine	WB	J Vet Med Sci; Protein phosphatase 6 regulates trametinib sensitivity, a mitogen-activated protein kinase kinase (MEK) inhibitor, by regulating MEK1/2-ERK1/2 signaling in canine melanoma cells; Authors - Miu Yamamoto ; KD Validated