PPP3R2 Polyclonal antibody

PPP3R2 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 14005-1-AP

Synonyms

Calcineurin B like protein, Calcineurin BII, Calcineurin subunit B type 2, CBLP, CNBII, PPP3R2, PPP3RL



Tested Applications

Positive WB detected inmouse testis tissue
Positive IP detected inmouse testis tissue
Positive IHC detected inhuman testis tissue, human prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14005-1-AP targets PPP3R2 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PPP3R2 fusion protein Ag5097
Full Name protein phosphatase 3 (formerly 2B), regulatory subunit B, beta isoform
Calculated Molecular Weight 20 kDa
Observed Molecular Weight 20 kDa
GenBank Accession NumberBC030595
Gene Symbol PPP3R2
Gene ID (NCBI) 5535
RRIDAB_2168590
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for PPP3R2 antibody 14005-1-APDownload protocol
IHC protocol for PPP3R2 antibody 14005-1-APDownload protocol
IP protocol for PPP3R2 antibody 14005-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Proc Natl Acad Sci U S A

SPATA33 localizes calcineurin to the mitochondria and regulates sperm motility in mice.

Authors - Haruhiko Miyata
mouseIHC

Cell Death Dis

miR-146a facilitates osteoarthritis by regulating cartilage homeostasis via targeting Camk2d and Ppp3r2.

Authors - Xudong Zhang
mouseIF, IHC, WB

FASEB J

Roles of glycogen synthase kinase 3 alpha and calcineurin in regulating the ability of sperm to fertilize eggs.

Authors - Souvik Dey
mouseWB

Eur J Pharmacol

Stachydrine hydrochloride suppresses phenylephrine-induced pathological cardiac hypertrophy by inhibiting the calcineurin/nuclear factor of activated T-cell signalling pathway.

Authors - Jiali Zheng