PPP2R2C Polyclonal antibody

PPP2R2C Polyclonal Antibody for WB, FC, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, FC, IHC, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 12747-1-AP

Synonyms

B55 GAMMA, IMYPNO, IMYPNO1, PP2A subunit B isoform gamma, PPP2R2C, PR52, PR55G

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Validation Data Gallery

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  WB analysis of HeLa using 12747-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12747-1-AP (PPP2R2C Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 12747-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 12747-1-AP (PPP2R2C antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 12747-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 12747-1-AP (PPP2R2C Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 12747-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 12747-1-AP (PPP2R2C antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human breast cancer using 12747-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 12747-1-AP (PPP2R2C antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human brain using 12747-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 12747-1-AP (PPP2R2C antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 12747-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 12747-1-AP (PPP2R2C antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human testis using 12747-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 12747-1-AP (PPP2R2C antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 12747-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 12747-1-AP (PPP2R2C antibody) at dilution of 1:50 (under 40x lens).

• 

  FC experiment of MCF-7 using 12747-1-AP

  1X10^6 MCF-7 cells were stained with 0.2ug PPP2R2C antibody (12747-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

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Tested Applications

• Positive WB detected in: HeLa cells, MCF-7 cells, mouse testis tissue
• Positive IHC detected in: human breast cancer tissue, human brain tissue, human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive FC detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below

Product Information

12747-1-AP targets PPP2R2C in WB, FC, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PPP2R2C fusion protein Ag3470
• Full Name: protein phosphatase 2 (formerly 2A), regulatory subunit B, gamma isoform
• Calculated Molecular Weight: 430 aa, 49 kDa
• Observed Molecular Weight: 49 kDa
• GenBank Accession Number: BC032954
• Gene Symbol: PPP2R2C
• Gene ID (NCBI): 5522
• RRID: AB_2170099
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB	Protein Cell; Senktide blocks aberrant RTN3 interactome to retard memory decline and tau pathology in social isolated Alzheimer's disease mice; Authors - He-Zhou Huang
human		J Cell Sci; The role of specific PP2A complexes in the dephosphorylation of γ-H2AX.; Authors - Liping Chen
mouse	WB	J Biochem; Involvement of PP2A Methylation in the Adipogenic Differentiation of Bone Marrow Derived Mesenchymal Stem Cell.; Authors - Shunta Ikeda
mouse	WB	Biochim Biophys Acta Mol Cell Res; Hsc70 phosphorylation patterns and calmodulin regulate AP2 CCV life span for cell adhesion protein transport; Authors - G F Sengül