PPP1R3C Polyclonal antibody

PPP1R3C Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 29424-1-AP

Synonyms

PP1 subunit R5, PPP1R3C, PPP1R5, Protein targeting to glycogen, PTG

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Validation Data Gallery

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  WB analysis using 29424-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29424-1-AP (PPP1R3C antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 29424-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 29424-1-AP (PPP1R3C antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 29424-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 29424-1-AP (PPP1R3C antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HEK-293T cells, mouse cerebellum tissue, rat cerebellum tissue
• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

29424-1-AP targets PPP1R3C in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PPP1R3C fusion protein Ag30280
• Full Name: protein phosphatase 1, regulatory (inhibitor) subunit 3C
• Calculated Molecular Weight: 317 aa, 36 kDa
• GenBank Accession Number: BC012625
• Gene Symbol: PPP1R3C
• Gene ID (NCBI): 5507
• RRID: AB_2918304
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

• Protein phosphatase 1 regulatory subunit 3C (PPP1R3C) is an enzyme that binds to protein phosphatase-1 (PP1) as a regulator. The central role of PPP1R3C is associated with glycogenesis and the overexpression of this gene increases glycogen storage. The cellular glycogen level is suppressed when PPP1R3C is knocked-down. PPP1R3C induces glycogen accumulation via HIF1 α during hypoxia and it is hypermethylated in melanoma (PMID: 25846879). Overexpression of PPP1R3C increased hepatic gluconeogenesis in vitro and in vivo while knockdown of PPP1R3C showed an opposite result (PMID: 31181215).