iASPP Polyclonal antibody

• Featured Product
• KD/KO Validated

iASPP Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat, mouse

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 18590-1-AP

Synonyms

IASPP, Inhibitor of ASPP protein, NFkB interacting protein 1, NKIP1, PPP1R13B like protein, PPP1R13BL, PPP1R13L, Protein iASPP, RAI, RelA associated inhibitor

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Validation Data Gallery

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  WB analysis using 18590-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18590-1-AP (iASPP antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of Apoptosised HeLa using 18590-1-AP

  Apoptosised HeLa cells were subjected to SDS PAGE followed by western blot with 18590-1-AP (iASPP antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-3 using 18590-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 18590-1-AP (iASPP antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 18590-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 18590-1-AP (iASPP antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IP experiment of PC-3 using 18590-1-AP

  IP result of anti-iASPP (IP:18590-1-AP, 4ug; Detection:18590-1-AP 1:1000) with PC-3 cells lysate 1040ug.

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  IHC staining of human breast cancer using 18590-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 18590-1-AP (iASPP antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 18590-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 18590-1-AP (iASPP antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of MCF-7 using 18590-1-AP

  Immunofluorescent analysis of MCF-7 cells, using PPP1R13L antibody 18590-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).Blue pseudocolor = DAPI (fluorescent DNA dye).

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Tested Applications

• Positive WB detected in: NIH/3T3 cells, PC-3 cells, MCF-7 cells, Apoptosised HeLa cells, C6 cells
• Positive IP detected in: PC-3 cells
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:400-1:1600
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 4 publications below
• IF: See 1 publications below
• IP: See 2 publications below

Product Information

18590-1-AP targets iASPP in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, rat, mouse samples.

• Tested Reactivity: human, rat, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: iASPP fusion protein Ag13273
• Full Name: protein phosphatase 1, regulatory (inhibitor) subunit 13 like
• Calculated Molecular Weight: 89 kDa
• Observed Molecular Weight: 110 kDa
• GenBank Accession Number: BC064913
• Gene Symbol: PPP1R13L
• Gene ID (NCBI): 10848
• RRID: AB_2168573
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Inhibitor of apoptosis-stimulating protein of p53 (iASPP), encoded by PPP1R13L gene, is often overexpressed in human cancers. The ASPP family includes three members, namely ASPP1, ASPP2, and iASPP, which are specific regulators of p53-, p63-, and p73-mediated apoptosis. ASPP1 and ASPP2 enhance the apoptotic function of p53, whereas iASPP specifically inhibits p53- mediated apoptosis. Overexpression of iASPP is associated with resistance to cisplatin-induced apoptosis and rediation therapy. iASPP plays a pivotal role in regulating cancer cell proliferation and tumor progression. This antibody could both recognize unphosphorylated and phosphorylated iASPP.

Publications

Species	Application	Title
human	WB,IP	J Cell Biol; iASPP contributes to cell cortex rigidity, mitotic cell rounding, and spindle positioning.; Authors - Aurélie Mangon ; KD Validated
human	WB,IP,IF	Cell Death Dis; iASPP-PP1 complex is required for cytokinetic abscission by controlling CEP55 dephosphorylation.; Authors - Kun Gao ; KD Validated
mouse	WB	J Biochem Mol Toxicol; Upregulation of iASPP ameliorates hypoxia/reoxygenation-induced apoptosis and oxidative stress in cardiomyocytes by upregulating Nrf2 signaling.; Authors - Baobao Bai ; KD Validated
mouse	WB	Acta Biochim Biophys Sin (Shanghai); iASPP protects the heart from ischemia injury by inhibiting p53 expression and cardiomyocyte apoptosis.; Authors - Timur Yagudin