PPM1A Polyclonal antibody

PPM1A Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA, Cell treatment

Conjugate

Unconjugated

Publications

3

Cat no : 12961-1-AP

Synonyms

PP2C ALPHA, PP2CA, PPM1A, PPPM1A, Protein phosphatase 1A, Protein phosphatase IA

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Validation Data Gallery

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  WB analysis using 12961-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12961-1-AP (PPM1A antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 12961-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12961-1-AP (PPM1A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 12961-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 12961-1-AP (PPM1A antibody) at dilution of 1:900 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 12961-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 12961-1-AP (PPM1A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 12961-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12961-1-AP (PPM1A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 12961-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12961-1-AP (PPM1A antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human urothelial carcinoma using 12961-1-AP

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 12961-1-AP (PPM1A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human urothelial carcinoma using 12961-1-AP

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 12961-1-AP (PPM1A antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: Jurkat cells, HepG2 cells, human liver tissue, rat kidney tissue, mouse kidney tissue, K-562 cells
• Positive IHC detected in: human breast cancer tissue, human urothelial carcinoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IF: See 1 publications below

Product Information

12961-1-AP targets PPM1A in WB, IF, IHC, ELISA, Cell treatment applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PPM1A fusion protein Ag3645
• Full Name: protein phosphatase 1A (formerly 2C), magnesium-dependent, alpha isoform
• Calculated Molecular Weight: 382 aa, 42 kDa
• Observed Molecular Weight: 42 kDa
• GenBank Accession Number: BC026691
• Gene Symbol: PPM1A
• Gene ID (NCBI): 5494
• RRID: AB_2169763
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PPM1A(Protein phosphatase 1A) is also named as PPPM1A and belongs to the PP2C family. It is a serine/threonine protein phosphatase that is essential for regulating cellular stress responses in eukaryotes. PPM1A plays an important role in tumor suppression and that it negatively controls many signaling pathways which are critical for trophoblast invasion.(PMID:19404668). This protein distributes in both the cytoplasm and nucleus of mammalian cells(PMID:9003755). It has 2 isoforms produced by alternative splicing.

Publications

Species	Application	Title
mouse	Cell treatment	Cell Rep; Blocking AMPK β1 myristoylation enhances AMPK activity and protects mice from high-fat diet-induced obesity and hepatic steatosis; Authors - Katyayanee Neopane
rat	IF	iScience; Induced retinal pigment epithelial cells with anti-epithelial-to-mesenchymal transition ability delay retinal degeneration; Authors - Haibin Tian
mouse	WB	Biol Pharm Bull; Sorghum (Sorghum bicolor) Extract-Induced Adipogenesis Is Independent of PPARγ Ser273 Phosphorylation in 3T3-L1 Adipocytes.; Authors - Aozora Nagaoka