BRN2 Polyclonal antibody

BRN2 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

5

Reviews (1)

Cat no : 14596-1-AP

Synonyms

Brain 2, Brn 2, BRN2, Oct 7, OCT7, Octamer binding protein 7, OTF 7, OTF7, POU class 3 homeobox 2, POU3F2, POUF3

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Validation Data Gallery

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  WB analysis of HeLa using 14596-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14596-1-AP (BRN2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 14596-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 14596-1-AP (BRN2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse brain using 14596-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14596-1-AP (BRN2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 14596-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14596-1-AP (BRN2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 14596-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 14596-1-AP (BRN2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 14596-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 14596-1-AP (BRN2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 14596-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 14596-1-AP (BRN2 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human placenta using 14596-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 14596-1-AP (BRN2 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human skin using 14596-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 14596-1-AP (BRN2 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human skin using 14596-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 14596-1-AP (BRN2 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human spleen using 14596-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 14596-1-AP (BRN2 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human spleen using 14596-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 14596-1-AP (BRN2 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human ovary using 14596-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 14596-1-AP (BRN2 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human ovary using 14596-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 14596-1-AP (BRN2 antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of mouse brain using 14596-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using BRN2 antibody (14596-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse brain using 14596-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using BRN2 antibody (14596-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of human gliomas using 14596-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human gliomas tissue using BRN2 antibody (14596-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, Jurkat cells
• Positive IHC detected in: mouse brain tissue, human gliomas tissue, human placenta tissue, human skin tissue, human spleen tissue, human ovary tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: mouse brain tissue, human gliomas tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IF: See 4 publications below

Product Information

14596-1-AP targets BRN2 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: BRN2 fusion protein Ag6131
• Full Name: POU class 3 homeobox 2
• Calculated Molecular Weight: 47 kDa
• Observed Molecular Weight: 47 kDa
• GenBank Accession Number: BC051699
• Gene Symbol: POU3F2
• Gene ID (NCBI): 5454
• RRID: AB_2167371
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

POU3F2 belongs to a large family of transcription factors that bind to the octameric DNA sequence ATGCAAAT. It shares a highly homologous region that referred to as the POU domain. POU3F2 is a Class III POU genes that expressed predominantly in the central nervous system (CNS). It is likely that CNS-specific transcription factors such as these play an important role in mammalian neurogenesis by regulating their diverse patterns of gene expression. POU3F2 is required for maintaining neural cell differetiation and has a role in the production and positioning of neocortical neurons.

Publications

Species	Application	Title
mouse	IF	Hum Mol Genet; Glutathione S-transferase Pi (Gstp) proteins regulate neuritogenesis in the developing cerebral cortex.; Authors - Xiaonan Liu
rat	WB	FEBS Lett; miR-221/222 suppression induced by activation of the cAMP/PKA/CREB1 pathway is required for cAMP-induced bidirectional differentiation of glioma cells.; Authors - Qian Liu
mouse	IF	Neurosci Lett; Overexpression of the 14-3-3gamma protein in embryonic mice results in neuronal migration delay in the developing cerebral cortex.; Authors - Brett Cornell
mouse	IF	Neuron; HDAC1 and HDAC2 Regulate Intermediate Progenitor Positioning to Safeguard Neocortical Development.; Authors - Tianxiang Tang
mouse	IF	Adv Sci (Weinh); Enabling Survival of Transplanted Neural Precursor Cells in the Ischemic Brain; Authors - Zhifu Wang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Mandi (Verified Customer) (09-26-2019)

Positive staining in human cortical neurons derived from iPSCs

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:500