POLR3D Monoclonal antibody

• Featured Product
• KD/KO Validated

POLR3D Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Rabbit

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

2F5G4

Cat no : 68263-1-Ig

Synonyms

BN51, BN51T, POLR3D, Protein BN51, RNA polymerase III subunit C4, RPC4, RPC53, RPC53 homolog, TSBN51

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Validation Data Gallery

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  WB analysis using 68263-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68263-1-Ig (POLR3D antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

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  WB analysis of rabbit brain using 68263-1-Ig

  rabbit brain tissue were subjected to SDS PAGE followed by western blot with 68263-1-Ig (POLR3D antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 68263-1-Ig

  WB result of POLR3D antibody (68263-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-POLR3D transfected HEK-293 cells.

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  WB analysis of K-562 using 68263-1-Ig

  K-562 cells were subjected to SDS PAGE followed by western blot with 68263-1-Ig (POLR3D antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: LNCaP cells, rabbit brain tissue, K-562 cells, HEK-293 cells, HeLa cells, Jurkat cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68263-1-Ig targets POLR3D in WB, ELISA applications and shows reactivity with Human, Rabbit samples.

• Tested Reactivity: Human, Rabbit
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: POLR3D fusion protein Ag7646
• Full Name: polymerase (RNA) III (DNA directed) polypeptide D, 44kDa
• Calculated Molecular Weight: 44 kDa
• Observed Molecular Weight: 44 kDa
• GenBank Accession Number: BC004484
• Gene Symbol: POLR3D
• Gene ID (NCBI): 661
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Polymerase (RNA) III (DNA directed) polypeptide D(POLR3D) plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses and acts as nuclear and cytosolic DNA sensor involved in innate immune response. POLR3D can sense non-self dsDNA that serves as template for transcription into dsRNA.