POLR2G Polyclonal antibody

POLR2G Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 18497-1-AP

Synonyms

hRPB19, hsRPB7, POLR2G, RNA polymerase II subunit B7, RPB19, RPB7

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Validation Data Gallery

• 

  WB analysis of A431 using 18497-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 18497-1-AP (POLR2G antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 18497-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18497-1-AP (POLR2G antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 18497-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 18497-1-AP (POLR2G antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A375 using 18497-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 18497-1-AP (POLR2G antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: A431 cells, A375 cells, Jurkat cells, HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

18497-1-AP targets POLR2G in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: POLR2G fusion protein Ag13338
• Full Name: polymerase (RNA) II (DNA directed) polypeptide G
• Calculated Molecular Weight: 172 aa, 19 kDa
• Observed Molecular Weight: 20-25 kDa
• GenBank Accession Number: BC112164
• Gene Symbol: POLR2G
• Gene ID (NCBI): 5436
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

POLR2G (DNA- directed RNA polymerase II subunit G), also known as hRPB19, hsRPB7 and RPB7, is the seventh largest subunit of RNA polymerase II. POLR2G functions in transcription initiation, and is also thought to help stabilize transcribing polyermase molecules during elongation.